CST, 14824S, TRF2 (P449) Antibody

Polyclonal Antibody for studying TRF2. Validated for Western Blotting. Highly specific and rigorously validated in-house, TRF2 (P449) Antibody (CST #14824) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRF2 (P449) Antibody recognizes endogenous levels of total TRF2 protein. This antibody also cross-reacts with an unidentified protein of 135 kDa. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro449 of human TRF2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Telomeres, the linear ends of chromosomes, are organized into T-loops to prevent them from being recognized by the cell as DNA double stranded breaks (DSBs) (1). The telomeric repeat binding factor proteins TRF1 and TRF2 bind to double-stranded telomeres to allow formation of T-loops (2). A large number of proteins involved in the DNA damage response are found physically associated with TRF2 within telomeres (3). Interestingly, TRF2 can transiently localize to DNA damage-induced DSBs, but overexpression of TRF2 prevents ATM-dependent signaling (4). Phosphorylation of TRF2 at Ser323 has been reported , but no upstream kinase or role has been established for this phosphorylation site (5). Alternate Names Telomeric DNA-binding protein; telomeric repeat binding factor 2; telomeric repeat binding protein 2; Telomeric repeat-binding factor 2; TERF2; TRBF2; TRF2; TTAGGG repeat-binding factor 2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit