CST, 14859S, Ikaros (D6N9Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ikaros. Validated for WB,WB,IHC,IF,IF,F,ChIP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, Ikaros (D6N9Y) Rabbit Monoclonal Antibody (CST #14859) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 CUT&RUN: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 30979 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT&RUN Specificity / Sensitivity Ikaros (D6N9Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total Ikaros protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro329 of human Ikaros protein. Background The Ikaros family of zinc-finger DNA-binding proteins belongs to the Kruppel transcription factor superfamily. Ikaros proteins are characterized by the presence of an amino-terminal zinc finger DNA-binding domain and a carboxy-terminal dimerization domain. Members of the Ikaros family include Ikaros, Aiolos, Helios, EOS, and Pegasus (1). All family members can form homodimers and heterodimers with other members of the Ikaros family. Most also contain multiple isoforms that are generated as a result of differential splicing, with some isoforms behaving in a dominant negative manner upon dimerization (2). Ikaros (IKZF1, LYF1) is the prototypical Ikaros family zinc-finger transcription factor and is expressed abundantly in lymphoid cells. Genetic studies in mice demonstrate that Ikaros is a tumor suppressor that is important for the normal development of B, T, natural killer, and dendritic cells (3,4). Additional studies show that imbalanced expression of different Ikaros isoforms, as well as mutations in the corresponding gene, can be associated with a number of hematologic malignancies in humans (2,5,6). Alternate Names CLL-associated antigen KW-6; CVID13; DNA-binding protein Ikaros; hIk-1; Hs.54452; IK1; IKAROS; Ikaros (zinc finger protein); IKAROS family zinc finger 1; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; IKZF1; LyF-1; LYF1; Lymphoid transcription factor LyF-1; PPP1R92; PRO0758; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros); ZNFN1A1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50-70 Source/Isotype: Rabbit IgG