CST, 14868S, Sec61A1 (D7Q6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SEC61A1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Sec61A1 (D7Q6V) Rabbit Monoclonal Antibody (CST #14868) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Sec61A1 (D7Q6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sec61A1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg273 of human Sec61A1 protein. Background Sec61 translocon is a channel complex located on the endoplasmic reticulum (ER) membrane to mediate membrane protein insertion into the organelle (1). There are three components in the complex, Sec61A, Sec61B, and Sec61G (2). Sec61A is the main component of the channel on the ER membrane and directly contacts nascent synthesized polypeptide TMD (transmembrane domain) for insertion (3). Sec61G functions in stablizing the channel (3). In addition to TMD insertion, Sec61 translocon has also been shown to be involved in ER calcium leakage (4,5). Both Bip and calmodulin can inhibit this leakage by their interaction with Sec61A (6,7). Sec61B has no obvious function related to target protein ER membrane insertion, but is involved in other vesicle trafficking processes such as EGFR and Her2 trafficking from the cytosol to nucleus (8,9), Gurken trafficking from Golgi to plasma membrane (10), and copper-transporting ATPase membrane distribution (11). Alternate Names HNFJ4; HSEC61; protein transport protein SEC61 alpha subunit; protein transport protein Sec61 subunit alpha; Protein transport protein Sec61 subunit alpha isoform 1; S61A1; SEC61; Sec61 alpha 1 subunit; Sec61 alpha 1 subunit (S. cerevisiae); Sec61 alpha-1; sec61 homolog; SEC61 translocon alpha 1 subunit; SEC61 translocon subunit alpha 1; SEC61A; SEC61A1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG