CST, 14895T, BCL6 (D4I2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCL6. Validated for WB,WB,IP,IF,F,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, BCL6 (D4I2V) Rabbit Monoclonal Antibody (CST #14895) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:1600 Chromatin IP: 1:100 CUT&RUN: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 47899 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN Specificity / Sensitivity BCL6 (D4I2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCL6 protein. This antibody is predicted to detect both human BCL6 isoform 1 and isoform 2. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment spanning residues Asp210 through Thr409 of human BCL6 protein. Background Chromosomal translocations result in misregulation of the proto-oncogene in patients with B cell-derived non-Hodgkin's lymphoma (1). The gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8). Alternate Names B cell CLL/lymphoma 6; B-cell CLL/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; BCL5; BCL6; BCL6 transcription repressor; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; zinc finger transcription factor BCL6S; ZNF51 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 87-98 Source/Isotype: Rabbit IgG