CST, 14896S, MIST1/bHLHa15 (D7N4B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying bHLHa15/MIST1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MIST1/bHLHa15 (D7N4B) Rabbit Monoclonal Antibody (CST #14896) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 65896 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity MIST1/bHLHa15 (D7N4B) Rabbit Monoclonal Antibody recognizes endogenous levels of total MIST1/bHLHa15 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln181 of human MIST1/bHLHa15 protein. Background Class A basic helix-loop-helix protein 15 (MIST1, bHLHa15) is a highly conserved basic helix loop helix family transcription factor that binds E-box motifs and regulates the expression of developmentally regulated genes (1). MIST1 can bind DNA as a homodimer, or may heterodimerize with other bHLH proteins to regulate target gene expression (1). MIST1 is expressed in an array of tissues, including salivary glands, stomach, small intestine, and the pancreas, but is generally restricted to secretory cell subtypes (2). In the pancreas, MIST1 is essential for the maturation, maintenance, and function of acinar cells (3). In gastric chief cells, MIST1 regulates the expression of RAB26 and RAB3D, two GTPases that function to regulate secretory granule formation (4). Loss of MIST1 in gastric chief cells may be a potential marker of gastric epithelial neoplasia (5). Alternate Names basic helix-loop-helix domain containing, class B, 8; basic helix-loop-helix family member a15; basic helix-loop-helix family, member a15; BHA15; BHLHA15; BHLHB8; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; class II bHLH protein MIST1; MIST-1; MIST1; Muscle, intestine and stomach expression 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG