CST, 14902S, SF2/ASF (D1N3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SF2/ASF. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SF2/ASF (D1N3S) Rabbit Monoclonal Antibody (CST #14902) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SF2/ASF (D1N3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SF2/ASF protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val172 of human SF2/ASF protein. Background SF2/ASF is a member of the Ser-Arg-rich (SR) protein family of highly conserved nuclear phosphoproteins involved in pre-mRNA splicing (1). Besides its role in nuclear pre-mRNA splicing, SF2/ASF has been shown to shuttle between the nucleus and cytoplasm, suggesting additional roles in mRNA transport and cytoplasmic events (2). SF2/ASF associates with translating ribosomes and stimulates translation (3). It also activates translation initiation by suppressing the activity of 4E-BP1, which is mediated by SF2/ASF association with mTOR and the phosphatase PP2A (4). More recent studies have demonstrated a role for SF2/ASF in microRNA processing (5). Alternate Names Alternative-splicing factor 1; ASF; ASF-1; MGC5228; pre-mRNA-splicing factor SF2, P33 subunit; serine and arginine rich splicing factor 1; Serine/arginine-rich splicing factor 1; SF2; SF2P33; SFRS1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1; SRp30a; SRSF1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG