CST, 14946S, MST1 (D8B9Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MST1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MST1 (D8B9Q) Rabbit Monoclonal Antibody (CST #14946) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MST1 (D8B9Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total MST1 protein. This antibody also detects the 37 kDa amino terminal fragment released by caspase cleavage. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro9 of human MST1 protein. Background Mammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple cellular processes, including proliferation, apoptosis, migration, and cytoskeletal rearrangement (1). This family of serine/threonine kinases includes MST1 (STK4) and MST2 (STK3), two functionally related proteins with conserved amino-terminal kinase domains and carboxy-terminal regulatory domains that contain nuclear export signals (1-3). During apoptosis, caspase-mediated cleavage of MST1/2 removes the inhibitory regulatory domain, triggering autophosphorylation and activation of the kinase domain, which is translocated to the nucleus. Nuclear translocation of the active kinase induces chromatin condensation and other events associated with apoptotic progression (4). Research studies indicate that MST1/2 are orthologous to Hippo (Hpo), one of the core regulatory proteins in the Hippo signaling pathway. This evolutionarily conserved program controls tissue growth and organ size by regulating cell proliferation, apoptosis, and stem cell self-renewal. The mammalian Hippo signaling pathway involves a kinase cascade, where the MST1/2 kinases and the SAV1 scaffold protein form a complex that leads to phosphorylation and activation of LATS1/2. The LATS1/2 kinases phosphorylate YAP and TAZ, promoting cytoplasmic sequestration and inhibition of these transcription coactivators (5). Alternate Names dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)); DKFZp686A2068; hippo homolog; kinase responsive to stress 2; KRS2; Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST-1; MST1; MST1/C; MST1/N; serine/threonine kinase 4; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase 4 18kDa subunit; Serine/threonine-protein kinase 4 37kDa subunit; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STK4; yeast Ste20-like; YSK3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG