CST, 14949T, TBC1D7 (D8K1Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TBC1D7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TBC1D7 (D8K1Y) Rabbit Monoclonal Antibody (CST #14949) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TBC1D7 (D8K1Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total TBC1D7 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TBC1D7 protein. Background TBC1 domain family member 7 (TBC1D7, TBC7) belongs to a family of TBC (Tre-2/Bub2/Cdc16) containing proteins that function as GTPase-activating proteins (GAPs) (1,2). TBC1D7 was initially identified as a novel binding protein within the TSC1-TSC2 complex, where it was thought to associate with TSC1 (3,4). Additional research indicates that TBC1D7 is a third subunit of the TSC1-TSC2 complex that possesses Rheb-GAP activity and signals upstream of mTORC1 (5). Knockdown of TBC1D7 limits the association between TSC1 and TSC2, resulting in reduced Rheb-GAP activity and increased mTORC1 signaling (5). Mutations in the corresponding gene result in increased mTORC1 signaling, delayed autophagy, and are associated with intellectual disability (ID) and macrocrania (6,7). Alternate Names cell migration-inducing protein 23; dJ257A7.3; DKFZp686N2317; FLJ32666; MGCPH; PIG51; TBC1 domain family member 7; TBC1 domain family, member 7; TBC1D7; TBC7; TBCD7; TS complex subunit 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG