CST, 14965T, Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CdcP1 (Tyr743) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit Monoclonal Antibody (CST #14965) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-CDCP1 (Tyr743) (D2G2J) Rabbit Monoclonal Antibody recognizes endogenous levels of CDCP1 protein only when phosphorylated at Tyr743. This antibody may cross-react with other tyrosine-phosphorylated proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr743 of human CDCP1 protein. Background CUB domain-containing protein 1 (CDCP1, SIMA135) is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative hematopoietic stem cell marker (4,5). Alternate Names CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases; TRASK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140, 70 Source/Isotype: Rabbit IgG