CST, 14966S, VPRBP (D5K5V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VPRBP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, VPRBP (D5K5V) Rabbit Monoclonal Antibody (CST #14966) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VPRBP (D5K5V) Rabbit Monoclonal Antibody recognizes endogenous levels of total VPRBP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1184 of human VPRBP protein. Background The HIV-1 viral protein R (Vpr)-binding protein (VPRBP, DCAF1) is a substrate-specific adaptor for the CUL4-based ubiquitin ligase complex that consists of CUL4A, RBX1, and DDB1 (1). VPRBP protein structure contains a central LIS1 homology (LisH) motif responsible for dimerization, and two carboxy-terminal WD-40 motifs involved in Vpr and DDB1 binding (2-4). Research studies demonstrate that VPRBP plays a role in hepatic lipid metabolism by promoting the ubiquitin-dependent proteasomal degradation of the TR4 nuclear receptor, which is involved in lipid homeostasis (5). The VPRBP protein plays a role in mammalian germ cell development through regulation of TET methylcytosine dioxygenase activation (6). Additional studies show that VPRBP exhibits kinase activity and phosphorylates histone H2A at Ser120, which blocks tumor suppressor gene transcription (7). The tumor suppressor Merlin/NF2 inhibits tumorigenesis through interaction with and suppression of the CUL4A-RBX1-DDB1-VPRBP complex (8). Alternate Names DCAF1; DDB1 and CUL4 associated factor 1; DDB1- and CUL4-associated factor 1; HIV-1 Vpr binding protein; HIV-1 Vpr-binding protein; KIAA0800; MGC102804; Protein VPRBP; RIP; Serine/threonine-protein kinase VPRBP; Vpr (HIV-1) binding protein; Vpr-binding protein; Vpr-interacting protein; VPRBP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Rabbit IgG