CST, 14979S, IRGM Antibody

Polyclonal Antibody for studying IRGM mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, IRGM Antibody (CST #14979) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRGM Antibody recognizes endogenous levels of total IRGM protein in mouse and rat. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg215 of mouse IRGM protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Immunity-related GTPase family M protein 1 (IRGM, LRG-47) belongs to the p47 family of immunity related guanosine triphosphatases (IRGs) that regulate innate immune responses to intracellular pathogens (1-3). Research studies indicate that IRGM plays a role in autophagy during clearance of intracellular bacteria (4). Expression of IRGM in mice, but not in humans, is induced by inflammatory signals that include interferon and LPS (2,3). Polymorphisms in the corresponding gene are associated with some cases of tuberculosis (5-7), Crohn's disease (8,9), and severe sepsis (10). Additional studies indicate that IRGM functions through regulation of autophagy (4). Mitochondrial IRGM plays a role in mitochondrial fission, membrane polarization, and mitophagy (11). Knockout mice for IRGM show increased susceptibility to infection as well as intestinal inflammation and Paneth cell abnormalities (12,13). Knockout mice against IRGM are also resistant to neuronal autophagy following stroke (14). RNA viruses commonly target IRGM in order to suppress autophagy and enhance infection (15). Alternate Names Ifggd3; Ifi1; Iigp3; Iipg3; immunity related GTPase m1; immunity-related GTPase family M member 1; Immunity-related GTPase family M protein; Immunity-related GTPase family M protein 1; immunity-related GTPase family, M; interferon inducible protein 1; interferon-gamma-inducible GTPase Ifggd3 protein; Interferon-inducible GTPase 3; Interferon-inducible protein 1; Irgm; Irgm1; LPS-stimulated RAW 264.7 macrophage protein 47; LRG-; LRG-47; OTTMUSP00000005664 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit