CST, 15083S, DMT1/SLC11A2 (D3V8G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DMT1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DMT1/SLC11A2 (D3V8G) Rabbit Monoclonal Antibody (CST #15083) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DMT1/SLC11A2 (D3V8G) Rabbit Monoclonal Antibody recognizes endogenous levels of total DMT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DMT1 protein. Background Divalent metal-ion transporter 1 (DMT1, SLC11A2, NRAMP2) is a transmembrane metal ion transport protein that plays critical roles in non-heme iron absorption in the intestine and iron acquisition by erythroid precursor cells (1,2). Following the cellular uptake of iron, DMT1 transfers ferric iron from the endosomes to the cytoplasm (3,4). The DMT1 protein can transport up to eight different metals, including iron, manganese, cobalt, and cadmium (5). Four mammalian DMT1 isoforms are expressed in various tissues and are differentially regulated at both the transcriptional and post-translational level (6,7). Mutations in the corresponding gene can result in hypochromic microcytic anemia and iron overload. Aberrant iron transport in these individuals results in erythroid hyperplasia, high serum iron, and impaired liver function (8-10). Research studies show elevated DMT1 levels and iron accumulation in the substantia nigra of Parkinson's disease patients and the corresponding animal model (11,12). Alternate Names AHMIO1; DCT1; Divalent cation transporter 1; Divalent metal transporter 1; DMT-1; DMT1; FLJ37416; Natural resistance-associated macrophage protein 2; NRAM2; NRAMP 2; NRAMP2; SLC11A2; solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2; Solute carrier family 11 member 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55, 70-100 Source/Isotype: Rabbit IgG