CST, 15099S, TRIM27 (D5S4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM27. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM27 (D5S4O) Rabbit Monoclonal Antibody (CST #15099) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRIM27 (D5S4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM27 protein. This antibody also cross-reacts with an unidentified protein of 80 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TRIM27 protein. Background Tripartite motif containing protein 27 (TRIM27, RFP) is a member of the tripartite motif (TRIM) family whose members contain a RING domain, a B-box, and a coiled-coil region (together called RBCC). TRIM27 was originally discovered as part of an oncogenic DNA rearrangement resulting in a fusion of the amino terminal RBCC region of TRIM27 with the carboxyl terminal kinase domain of the receptor tyrosine kinase Ret (1). Overexpression of TRIM27 induces JNK and p38 MAPK activation as well as apoptosis (2). TRIM27 has been found to have pleiotropic effects including transcriptional repression (3,4), and E3 ligase activity for ubiquitin (5-7), and SUMO (8). TRIM27 was originally found to interact with Enhancer of Polycomb (EPC) and function as a transcriptional repressor (3). Subsequent studies have identified ubiquitin E3 ligase activity in TRIM27 as well as other members of the TRIM family (reviewed in 9). Potential substrates of TRIM27-mediated ubiquitination include class II PI3K-C2β, NOD2, and WASH. Elevated expression of TRIM27 has been observed in several types of cancer, where in some cases it may be a predictor of poor prognosis (10-13). Alternate Names Ret finger protein; RFP; RFP transforming protein; RING finger protein 76; RING-type E3 ubiquitin transferase TRIM27; RNF76; TRI27; TRIM27; tripartite motif containing 27; tripartite motif protein TRIM27; tripartite motif-containing 27; Tripartite motif-containing protein 27; Zinc finger protein RFP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG