CST, 15129S, RGS4 (D4V1P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RGS4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RGS4 (D4V1P) Rabbit Monoclonal Antibody (CST #15129) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RGS4 (D4V1P) Rabbit Monoclonal Antibody recognizes endogenous levels of total RGS4 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human RGS4 protein. Background Regulators of G protein signaling (RGS) are a family of about 40 proteins that determine the signaling amplitude and duration of G protein-coupled receptor signaling via modulation of the GTPase activity of G proteins (1-3). Each RGS has a distinct structure, expression pattern, and regulation, resulting in preferential interactions with receptors, G proteins, and other signaling proteins, as well as a unique function (4). Regulator of G-protein signaling 4 (RGS4) is a small RGS (23 kDa) that belongs to the R4 subfamily of heterotrimeric G-protein regulators. RGS4 contains a short amino-terminus that may be responsible for its functional specificity towards G-protein-coupled receptors, and a conserved RGS domain responsible for GAP activity (1). The RGS4 protein shares activity at both Gαq and Gαi/o proteins (5,6). Degradation of RGS4 is regulated by opioid receptor-mediated ubiquitination following stimulation by opioid receptor agonists, such as the DAMGO synthetic opioid peptide (7). RGS4 modulates the action of morphine and other opiates (8). Alternate Names DKFZp761F1924; MGC2124; MGC60244; regulator of G protein signaling 4; Regulator of G-protein signaling 4; regulator of G-protein signalling 4; RGP4; RGS4; schizophrenia disorder 9; SCZD9 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG