CST, 15178T, Myeloperoxidase (E2Z8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MPO. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Myeloperoxidase (E2Z8J) Rabbit Monoclonal Antibody (CST #15178) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Myeloperoxidase (E2Z8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total myeloperoxidase protein. This antibody recognizes the full-length and heavy chain subunits of human and mouse myeloperoxidase protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myeloperoxidase protein. Background Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1). Alternate Names 84 kDa myeloperoxidase; 89 kDa myeloperoxidase; MPO; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 60, 80-90 Source/Isotype: Rabbit IgG