CST, 15218T, TFAM (F5A3U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TFAM mouse. Validated for WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, TFAM (F5A3U) Rabbit Monoclonal Antibody (CST #15218) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 16503 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TFAM (F5A3U) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse TFAM protein. A weak band is detected at 29 kDa that is thought to be non-specific. Non-specific staining of mouse testis was observed by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TFAM protein. Background TFAM (Transcription Factor A, Mitochondrial; aka TCF6) is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. TFAM is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B (1). Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is critical for normal health. TFAM plays an essential role in the maintenance of mtDNA and thus, ATP production (2). TFAM binds to mtDNA both nonspecifically and in a sequence-specific manner. It is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA (3). TFAM attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia (4). Alternate Names AI661103; Hmgt; Hmgts; mtTF; mtTFA; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; Tfam; Transcription factor A, mitochondrial; TS-HMG; tsHM; tsHMG Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG