CST, 15265S, Tox4 Antibody

Polyclonal Antibody for studying Tox4. Validated for Western Blotting. Highly specific and rigorously validated in-house, Tox4 Antibody (CST #15265) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tox4 Antibody recognizes endogenous levels of total Tox4 protein. This antibody does not cross-react with human Tox, Tox2, or Tox3 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln69 of human Tox4 protein. Antibodies are purified by peptide affinity chromatography. Background Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox4 is a regulatory factor of PP1 phosphatases and plays important roles in transcription, which include restricting pause release and early productive elongation, facilitating transcriptional reinitiation, and promoting late productive elongation (3). Tox4 has been shown to modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (4). Tox4 is a regulator of hepatic glucose production in an insulin receptor and FoxO1-independent manner, and its expression is increased in the liver in diabetic patients with nonalcoholic fatty liver disease (NAFLD) (5). Alternate Names C14orf92; Epidermal Langerhans cell protein LCP1; KIAA0737; LCP1; MIG7; migration-inducing protein 7; TOX high mobility group box family member 4; TOX4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit