CST, 15446S, Phospho-Jak2 (Ser523) (F8E8U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jak2 (Ser523) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Jak2 (Ser523) (F8E8U) Rabbit Monoclonal Antibody (CST #15446) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Jak2 (Ser523) (F8E8U) Rabbit Monoclonal Antibody recognizes endogenous levels of Jak2 protein only when phosphorylated at Ser523. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human Jak2 protein. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. The JH2 domain of Jak2 has been shown to autophosphorylate two negative regulatory sites on Jak2, Ser523 and Tyr570. In the absence of cytokine stimulation, Jak2 is constitutively phosphorylated on Ser523, effectively inhibiting its activity (6,7). Mutation in the JH2 domain can lead to an increase in Jak2 activity, resulting in myeloproliferative neoplasms (MPNs) (8). Alternate Names JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG