CST, 15454SF, IFN-beta1 (D2J1D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IFN-beta1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, IFN-beta1 (D2J1D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #15454) is ready to ship. Product Usage Information This product is the carrier free version of product #97450. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 97450 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IFN-beta1 (D2J1D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IFN-β1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant mouse IFN-β1 protein. Background The type I interferon (IFN) family includes IFN-β1 and IFN-α1 through IFN-α13 in humans and IFN-α1 through IFN-α14 in mice. Type I IFN is produced following detection of pathogen-associated molecular patterns (PAMPs) and is important for induction of antiviral genes, activation of dendritic cells, and initiation of adaptive immunity (1, 2). Type I IFNs signal through the IFN alpha receptor (IFNAR), which is a heterodimer composed of IFNAR1 and IFNAR2. Activation of IFNAR leads to formation of the nuclear complex IFN-stimulated gene factor 3 (ISGF3), which consists of STAT1, STAT2, and IRF-9 (3, 4). ISGF3 binds to IFN-stimulated response elements (ISREs) to initiate transcription of interferon-stimulated genes (3). Alternate Names Ifb; IFN-b; IFN-beta; Ifnb; Ifnb1; Interferon beta; interferon beta 1, fibroblast Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 24, 26 Source/Isotype: Rabbit IgG