CST, 15518SF, CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD34 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #15518) is ready to ship. Product Usage Information This product is the carrier free version of product #26233. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 26233 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD34 protein. Nuclear background may be observed in cultured cell lines by immunofluorescence. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD34 recombinant protein. Background CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts (1). CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention (2). The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion (3). CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34 (1,4). Alternate Names AU040960; Cd34; CD34 antigen; cluster designation 34; Hematopoietic progenitor cell antigen CD34 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 80-120 Source/Isotype: Rabbit IgG