CST, 15585S, CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PECAM-1 mouse. Validated for WB,WB,IF,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (CST #15585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Frozen): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD31 (PECAM-1) protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD31 (PECAM-1) protein. Background CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). Alternate Names C85791; Cd31; MGC102160; OTTMUSP00000003452; OTTMUSP00000003454; PECA1; Pecam; PECAM-1; PECAM-1/CD31; Pecam1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG