CST, 15588SF, TIAR (D32D3) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TIAR. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, TIAR (D32D3) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #15588) is ready to ship. Product Usage Information This product is the carrier free version of product #8509. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8509 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TIAR (D32D3) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TIAR protein. This antibody also weakly recognizes recombinant TIA1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIAR protein. Background TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6). Alternate Names aging-associated gene 7 protein; MGC33401; Nucleolysin TIAR; T-cluster binding protein; TCBP; TIA-1 related protein; TIA-1-related nucleolysin; TIA-1-related protein; TIA1 cytotoxic granule associated RNA binding protein like 1; TIA1 cytotoxic granule-associated RNA binding protein-like 1; TIA1 cytotoxic granule-associated RNA-binding protein-like 1; TIA1 related; TIAL1; TIAR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG