CST, 16323T, FANCD2 (D5L5X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FANCD2 isoform 2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, FANCD2 (D5L5X) Rabbit Monoclonal Antibody (CST #16323) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FANCD2 (D5L5X) Rabbit Monoclonal Antibody recognizes endogenous levels of total FANCD2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly995 of human FANCD2 protein. Background Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). In response to DNA damage, the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM) induces mono-ubiquitination of FANCD2 and FANCI (2).Monoubiquitination of FANCD2 induces localization of FANCD2 to sites of DNA damage, where it interacts with BRCA1. FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are also recruited to sites of DNA damage (3). Alternate Names DKFZp762A223; FA complementation group D2; FA-D2; FA4; FACD; FACD2; FAD; FAD2; FANCD; FANCD2; Fanconi anemia complementation group D2; Fanconi anemia group D2 protein; Fanconi anemia, complementation group D2; FLJ23826; Protein FACD2; type 4 Fanconi pancytopenia Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 155, 162 Source/Isotype: Rabbit IgG