CST, 16356S, Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3116)

Monoclonal Antibody for studying Catenin-beta (Thr41/Ser45) non-phosphate. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3116) (CST #16356) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser45 of human β-catenin protein. Background β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser45. Non-phospho β-Catenin (Ser45) protein is useful as a readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway (9). Alternate Names armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG