CST, 16435S, HCoV-OC43 Spike Protein (E4U6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HCoV-OC43 Spike glycoprotein HCoVOC43. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HCoV-OC43 Spike Protein (E4U6P) Rabbit Monoclonal Antibody (CST #16435) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HCoV-OC43 Spike Protein (E4U6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total HCoV-OC43 spike protein. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of HCoV-OC43 spike protein. Background Seasonal coronaviruses are a group of viruses that cause mild to moderate upper respiratory tract infections in humans. These viruses are part of the broader coronavirus family, which includes both mild strains and more severe strains like the one responsible for the COVID-19 pandemic (SARS-CoV-2). The most common seasonal coronaviruses include HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43. Similar to the others, HCoV-OC43 can cause mild upper respiratory tract infections. It's been known to circulate for a long time and is associated with cold-like symptoms, particularly in the winter months (1). The HCoV-OC43 spike protein is responsible for recognizing and binding to specific receptors on the surface of host cells. These receptors are believed to be N-acetyl-9-O-acetylneuraminic acid or N-glycolylneuraminic acid. Binding of HCoV-OC43 spike protein to these receptors initiates the process of viral entry into the host cell. After binding to the receptor on the host cell surface, the HCoV-OC43 spike protein undergoes structural changes that facilitate the fusion of the viral envelope with the host cell membrane. This fusion allows the viral genetic material to enter the host cell, initiating the viral replication process (1,2). Alternate Names E2; Peplomer protein; S glycoprotein; Spike glycoprotein; Spike protein S1; Spike protein S2; spike surface glycoprotein; SPIKE_CVHOC Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 200 Source/Isotype: Rabbit IgG