CST, 16439S, NKX6.1 (D8O4R) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying NKX6-1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, NKX6.1 (D8O4R) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #16439) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity NKX6.1 (D8O4R) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human NKX6.1 protein. Background The homeodomain protein NKX6.1 is a transcription factor that regulates pancreatic β-cell development (1). Overexpressed NKX6.1 stimulates rat pancreatic β-cell proliferation and increases glucose-stimulated insulin secretion (GSIS) (2). The effect on GSIS was shown to be mediated by the upregulation of prohormone VGF expression and the subsequent potentiation by TLQP-21, a peptide derived from VGF (3). Both nuclear receptors Nr4a1 and Nr4a3 are essential for pancreatic β-cell proliferation driven by overexpressed NKX6.1 (4). In addition, studies suggest that NKX6.1 is a suppressor for epithelial-mesenchymal transition (EMT), leading to inhibition of tumor metastasis (5). Alternate Names Homeobox protein NK-6 homolog A; Homeobox protein Nkx-6.1; NK homeo box, family 6, member A; NK homeobox, family 6, A; NK6 homeobox 1; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1; NKX6-1; NKX6.1; NKX61; NKX6A Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG