CST, 16503SF, TFAM (F5A3U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TFAM mouse. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, TFAM (F5A3U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #16503) is ready to ship. Product Usage Information This product is the carrier free version of product #15218. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15218 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TFAM (F5A3U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total mouse TFAM protein. A weak band is detected at 29 kDa that is thought to be non-specific. Non-specific staining of mouse testis was observed by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TFAM protein. Background TFAM (Transcription Factor A, Mitochondrial; aka TCF6) is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. TFAM is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B (1). Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is critical for normal health. TFAM plays an essential role in the maintenance of mtDNA and thus, ATP production (2). TFAM binds to mtDNA both nonspecifically and in a sequence-specific manner. It is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA (3). TFAM attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia (4). Alternate Names AI661103; Hmgt; Hmgts; mtTF; mtTFA; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; Tfam; Transcription factor A, mitochondrial; TS-HMG; tsHM; tsHMG Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG