CST, 16563T, FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD19 Anti-Idiotype Ab. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody (CST #16563) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of CARs containing an FMC63-derived scFv. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with an scFv derived from a murine anti-CD19 monoclonal antibody, FMC63. Background Chimeric antigen receptor (CAR) T cell therapy utilizes synthetic receptors to redirect T cells to bind and eliminate tumor cells, bypassing traditional T cell receptor (TCR) signaling (1). The extracellular domain of conventional CARs consists of single-chain variable fragments (scFvs), which recognize and bind target antigens (1). CARs engineered to express the scFv of a murine anti-CD19 antibody, FMC63, have shown clinical success in targeting CD19-expressing malignant B cells for degradation. This clinical success has led to several FMC63-based CARs being approved by the U.S. Food and Drug Administration (FDA) for the treatment of B-cell leukemia and lymphoma (2,3). Alternate Names B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12 Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG