CST, 16611T, Bcl-3 (E4D4K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCL3. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Bcl-3 (E4D4K) Rabbit Monoclonal Antibody (CST #16611) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Bcl-3 (E4D4K) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bcl-3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human Bcl-3 protein. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). Bcl-3 is an atypical member of the IκB family. Unlike the classic IκB family members, Bcl-3 associates with p50/NF-κB1 or p52/NF-κB2 homodimers in nuclei and modulates transcription in a context-dependent manner (9,10). It is also involved in a recurring translocation, t(14;19)(q32;q13), found in some patients with B-cell chronic lymphocytic leukemia (B-CLL) and lymphoma (11,12). Alternate Names B cell CLL/lymphoma 3; B-cell CLL/lymphoma 3; B-cell leukemia/lymphoma 3; B-cell lymphoma 3 protein; B-cell lymphoma 3-encoded protein; BCL-3; BCL3; BCL3 transcription coactivator; BCL4; chronic lymphatic leukemia protein; D19S37; Proto-oncogene BCL3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG