CST, 16709T, Cleaved Caspase-8 (Asp387) (p10 subunit) (E8K5S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp8 (Asp387) (p10) cleaved mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp387) (p10 subunit) (E8K5S) Rabbit Monoclonal Antibody (CST #16709) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Cleaved Caspase-8 (Asp387) (p10 subunit) (E8K5S) Rabbit Monoclonal Antibody recognizes endogenous levels of the p10 subunit of cleaved caspase-8 protein only when cleaved at Asp387. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein. Background Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. In addition to functioning as a key initiator caspase for extrinsic apoptosis, more recent studies have identified caspase-8 as a regulator of inflammatory necrotic cell death pathways such as necroptosis and pyroptosis (4,5). Activation of caspase-8 leads to cleavage of RIPK1 and RIPK3 to inhibit necroptosis (6,7). As a result, caspase-8 deficiency in mice, which is embryonic lethal, can be rescued by deletion of the necroptosis proteins RIPK3 or MLKL (8-11). Additionally, in some circumstances, caspase-8 is recruited to the inflammasome to trigger pyroptosis (12,13). Studies have also found that expression of an enzymatically inactive form of caspase-8 (C362S) causes embryonic lethality by inducing necroptosis and pyroptosis (14). Alternate Names Casp; CASP-8; Casp8; caspase 8; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; Fas-linked ICE-like protease; FLI; FLICE; MAC; MACH; Mch5 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG