CST, 16983SF, Nestin (Rat-401) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying nestin rat. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Nestin (Rat-401) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #16983) is ready to ship. Product Usage Information This product is the carrier free version of product #4760. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4760 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Nestin (Rat-401) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of nestin protein. Species Reactivity: Rat Source / Purification Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord. Background Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly downregulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, and astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous. Alternate Names Nes; NEST; Nestin Specification REACTIVITY: R SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1