CST, 16992T, Fc-epsilon RIA (E6D9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FCER1A mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Fc-epsilon RIA (E6D9L) Rabbit Monoclonal Antibody (CST #16992) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Fc-epsilon RIA (E6D9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcεRIA protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse FcεRIA recombinant protein. Background FcεRI, the high affinity IgE receptor complex, is made up of an α-chain, a β-chain, and a homodimer of two disulphide-linked γ-chains (1-4). It is expressed by mast cells, basophils, and antigen-presenting cells (1-4). The α-chain, FcεRIA, binds the Fc portion of IgE, which initiates antigenic crosslinking and leads to an allergic response, resulting in secretion of molecules such as interleukin-4 (IL-4), interleukin-6 (IL-6), tumor-necrosis factor-α (TNF-α), and granulocyte-macrophage colony-stimulating factor (GM-CSF) (1-5). Alternate Names Fc receptor, IgE, high affinity I, alpha polypeptide; Fc-epsilon RI-alpha; FcE; Fce1; Fce1a; fcepsilonri; Fcer1a; FCERA; FcERI; Fcr-; Fcr-5; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 45-65 Source/Isotype: Rabbit IgG