CST, 17012S, SLPI (F6Z7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLPI. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SLPI (F6Z7H) Rabbit Monoclonal Antibody (CST #17012) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SLPI (F6Z7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLPI protein. This antibody detects an 85 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe30 of human SLPI protein. Background Secretory leukocyte protease inhibitor (SLPI), also known as antileukoproteinase, is a small protein expressed primarily at mucosal surfaces (1), where it helps to protect against inflammation by inhibiting proteases, including elastase, chymotrypsin, and cathepsin G (2,3). SLPI also exerts antimicrobial activity against various Gram-positive and Gram-negative bacteria, some fungi and viruses, and suppresses the production of pro-inflammatory cytokines through NF-kB inhibition (1,4). SLPI has recently been shown to be upregulated in cancer-associated fibroblasts, and extracellular vesicle-encapsulated SLPI levels were reported to be correlated with tumor stage in ovarian cancer patients (5). Higher SLPI expression levels are also correlated with poorer prognosis in other cancer types, including colorectal and breast cancer (6,7). SLPI is essential for normal granulocytic differentiation and may be of therapeutic interest for congenital neutropenia patients (8). Alternate Names ALK1; ALP; Antileukoproteinase; BLPI; HUSI; HUSI-1; HUSI-I; MPI; mucus proteinase inhibitor; OTTHUMP00000031775; P03973; Protease inhibitor WAP4; secretory leukocyte peptidase inhibitor; Secretory leukocyte protease inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); seminal proteinase inhibitor; SLPI; WAP four-disulfide core domain 4; WAP four-disulfide core domain protein 4; WAP4; WFDC4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG