CST, 17346S, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3059)

Monoclonal Antibody for studying Jagged1. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3059) (CST #17346) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3059) recognizes endogenous levels of total Jagged1 protein. Based on sequence analyses, this antibody conjugate is not predicted to detect Jagged2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1131 of human Jagged1 protein. Background Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). Alternate Names AGS; AGS1; AHD; AWS; CD339; DCHE; hJ1; JAG1; jagged 1 (Alagille syndrome); jagged canonical Notch ligand 1; Jagged1; JAGL1; MGC104644; Protein jagged-1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG