CST, 17384S, V5-Tag (D3H8Q) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, V5-Tag (D3H8Q) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #17384) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:800 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity V5-Tag (D3H8Q) Rabbit mAb detects recombinant proteins containing the V5 epitope tag. The antibody recognizes the V5-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. V5-Tag (D3H8Q) Rabbit mAb detects exogenously expressed V5-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5 tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG