CST, 17460S, DCC (E4U1X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DCC. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, DCC (E4U1X) Rabbit Monoclonal Antibody (CST #17460) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DCC (E4U1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total DCC protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1200 of human DCC protein. Background Deleted in colorectal cancer (DCC) is a type I transmembrane protein belonging to the immunoglobulin superfamily. Originally implicated as a tumor suppressor gene given its lack of expression in colorectal carcinomas, DCC was later characterized as a receptor for netrin (1). Netrin proteins belong to an evolutionarily conserved family of laminin-like molecules that play an essential role in axon guidance during development. These secreted proteins can have opposing functions depending on specific receptor associations. Binding of netrin-1 by DCC on commissural axons mediates growth cone attraction, while binding by uncoordinated 5 (UNC5) family receptors induces repulsion (2,3). In addition to axon guidance, DCC is also suggested to regulate tissue organization, cell adhesion, motility, proliferation, differentiation, and cell survival via its binding activity with netrin-1 (1,3). Recent findings suggest that reduced expression of netrin-1 or DCC can lead to dopaminergic (DA) neuron death within the substantia nigra pars compacta (SNpc) of the midbrain, an area that is particularly vulnerable to degeneration in Parkinson's disease (PD). Conversely, overexpression of netrin-1 in animal models of PD has been shown to be neuroprotective, suggesting that the netrin-1/DCC ligand/receptor pair may be a therapeutic target for this neurodegenerative disease (4,5). Alternate Names Colorectal cancer suppressor; colorectal tumor suppressor; CRC18; CRCR1; DCC; DCC netrin 1 receptor; deleted in colorectal cancer protein; deleted in colorectal carcinoma; HGPPS2; IGDCC1; Immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; MRMV1; Netrin receptor DCC; NTN1R1; putative colorectal tumor suppressor; Tumor suppressor protein DCC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 185 Source/Isotype: Rabbit IgG