CST, 17519S, CR1/CD35 (E8B1Z) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying CR1. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CR1/CD35 (E8B1Z) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #17519) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CR1/CD35 (E8B1Z) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CR1/CD35 protein. Background Complement receptor type 1 (CR1/CD35) is a type I transmembrane glycoprotein that is expressed on the surface of B cells, neutrophils, monocytes, and renal podocytes (1,2). As a component of the host innate immune system, CR1/CD35 expressed on neutrophils and monocytes binds to ligands coated with the complement opsonins, C3b and C4b, which facilitates phagocytosis and production of proinflammatory cytokines (3,4). CR1/CD35 also participates in negative regulation of the complement cascade through its ability to promote dissociation of C3 and C5 convertases (4,5) and by serving as one of multiple cofactors for factor-I-mediated cleavage and inactivation of C3b and C4b (6). Alternate Names C3-binding protein; C3b/C4b receptor; C3BR; C4BR; CD35; CD35 antigen; complement C3b/C4b receptor 1 (Knops blood group); complement component (3b/4b) receptor 1 (Knops blood group); complement component receptor 1; complement receptor 1; Complement receptor type 1; CR1; KN; Knops blood group antigen Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG