CST, 17545S, TPBG/5T4 (E3M5R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TPBG. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, TPBG/5T4 (E3M5R) Rabbit Monoclonal Antibody (CST #17545) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TPBG/5T4 (E3M5R) Rabbit Monoclonal Antibody recognizes endogenous levels of total TPBG/5T4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TPBG/5T4 protein. Background Trophoblast glycoprotein (TPBG/5T4) is a type I transmembrane glycoprotein that is normally expressed by trophoblast cells of the placenta with a very limited expression pattern in normal adult tissues (1,2). The extracellular domain of TPBG is extensively glycosylated and contains multiple leucine-rich repeats while its cytoplasmic domain consists of a PDZ domain-binding motif, which is important for linking TPBG to intracellular signaling networks involved in the regulation of cell motility and adhesion (3,4). Research studies have shown that cell surface expression of TPBG plays a critical role in modulating signaling cascades that drive cell adhesion, morphology, and motility processes that are fundamental for normal progression of embryogenesis (5-7). Research studies have demonstrated that TPBG is aberrantly overexpressed in numerous types of solid tumors (8) and functions to promote enhanced tumor cell motility and metastasis (9,10). In some tumors, such as NSCLC and HNSCC, TPBG has been identified as a novel marker of tumor-initiating cells (11,12). The observed differential expression of TPBG by normal tissue versus tumor tissue has been exploited by multiple immunotherapeutic agents that are currently being evaluated for targeting of multiple types of solid tumors (13). Alternate Names 5T4; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4AG; M6P1; Oncofetal antigen 5T4; TPBG; Trophoblast glycoprotein; WAIF1; Wnt-activated inhibitory factor 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG