CST, 17758SF, Podoplanin (D9D7) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Podoplanin. Validated for Western Blotting,Flow Cytometry (Live). Highly specific and rigorously validated in-house, Podoplanin (D9D7) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #17758) is ready to ship. Product Usage Information This product is the carrier free version of product #9047. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9047 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Podoplanin (D9D7) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total podoplanin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp31 of human podoplanin protein. Background Podoplanin (aggrus, glycoprotein 36) is a single-pass transmembrane protein belonging to the type-1 family of sialomucin-like glycoproteins. Podoplanin was first described in the rat as a surface glycoprotein that regulated podocyte morphology (1). It is now commonly used as a marker of lymphatic endothelial cells, where its expression is associated with the process of lymphangiogenesis (2). Its role in this regard is presumably due to its putative involvement in regulating actin cytoskeleton dynamics (3). Research studies have shown that podoplanin expression is upregulated in a number of tumor types including colorectal cancers (4), oral squamous cell carcinomas (5), and germ cell tumors (6), with higher expression levels often associated with more aggressive tumors (7). Research studies have suggested a functional role for podoplanin in the stromal microenvironment of tumors. For example, it has been reported that podoplanin expression in cancer-associated fibroblasts (CAFs) is positively associated with a stromal environment that promotes cancer progression (8,9). Alternate Names 29kDa cytosolic podoplanin intracellular domain; Aggrus; Glycoprotein 36; glycoprotein, 36-KD; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS8; PA2.26; PA2.26 antigen; PDPN; PICD; Podoplanin; T1-alpha; T1A; T1A-2; T1A2; TI1A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG