CST, 17772S, Phospho-Lamin B1 (Ser210) Antibody

Polyclonal Antibody for studying Lamin B1 (Ser210) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Lamin B1 (Ser210) Antibody (CST #17772) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Lamin B1 (Ser210) Antibody recognizes endogenous levels of lamin B1 protein only when phosphorylated at Ser210. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser210 of human lamin B1 protein. Antibodies are purified by peptide affinity chromatography. Background Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene . Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10). Phosphorylation of lamin B1 at Ser210 in the rod domain by the combined activities of the autophagy kinase ULK1 and the mitotic kinases Aurora A and PLK1 contributes to mitotic spindle maintenance. For more information, visit Biorxiv at biorxiv.org/content/10.1101/2025.04.25.650690v1. Alternate Names ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; LMNB1; MGC111419 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 68 SOURCE: Rabbit