CST, 17805S, IRG1 Antibody

Polyclonal Antibody for studying IRG1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, IRG1 Antibody (CST #17805) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRG1 Antibody recognizes endogenous levels of total IRG1 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IRG1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconitic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6). Alternate Names Acod1; Aconitate decarboxylase; Aconitate decarboxylase 1; AI323667; CAD; Cis-aconitate decarboxylase; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; immunoresponsive gene 1; Irg1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit