CST, 18023S, ATP5A1 Antibody

Polyclonal Antibody for studying ATP5A1. Validated for Western Blotting. Highly specific and rigorously validated in-house, ATP5A1 Antibody (CST #18023) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity ATP5A1 Antibody recognizes endogenous levels of total ATP5A1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu209 of human ATP5A1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background ATP synthase alpha-subunit (ATP5A1) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. Generation of ATP is catalyzed by ATP synthase. ATP synthase is composed of a soluble catalytic core, F , and a membrane-spanning proton channel, F . ATP5A1 is one of five different subunits that comprise the catalytic F core. Interestingly, ATP5A1 is genetically linked to neurodevelopmental diseases (1). Moreover, the genetically linked mutant forms of , the most common genetic causes of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), bind to ATP5A1 and alter its protein expression, suggesting that mitochondrial dysfunction may be a major driver of disease in C9ORF72-linked ALS/FTD (2). Alternate Names ATP synthase alpha chain, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5A; ATP5A1; ATP5AL2; ATP5F1A; ATPA; ATPM; COXPD22; epididymis secretory sperm binding protein Li 123m; hATP1; HEL-S-123m; MC5DN4; mitochondrial ATP synthetase, oligomycin-resistant; MOM2; OMR; ORM Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 54 SOURCE: Rabbit