CST, 18030S, PC1/3 Antibody

Polyclonal Antibody for studying PCSK1. Validated for Western Blotting. Highly specific and rigorously validated in-house, PC1/3 Antibody (CST #18030) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity PC1/3 Antibody recognizes endogenous levels of total PC1/3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg364 of human PC1/3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3). Research studies examining the gene (encoding PC1/3) suggest that mutations that cause impaired PC1/3 expression or enzymatic activity contribute to obesity (4). Alternate Names BMIQ12; NEC 1; NEC1; Neuroendocrine convertase 1; PC1; PC3; PCSK1; Prohormone convertase 1; prohormone convertase 3; Proprotein convertase 1; proprotein convertase subtilisin/kexin type 1; SPC3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit