CST, 18163S, RPL11 (D1P5N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RPL11. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RPL11 (D1P5N) Rabbit Monoclonal Antibody (CST #18163) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RPL11 (D1P5N) Rabbit Monoclonal Antibody recognizes endogenous levels of total RPL11 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RPL11 protein. Background Ribosomal protein L11 (RPL11) is a nucleolar protein and component of the 60S ribosomal subunit. Research studies have shown that RPL11 plays a critical role in eukaryotic ribosome biogenesis (1). It has also been suggested that extraribosomal RPL11 functions in an RP-MDM2-p53 network in which RPL11 acts as a sensor of nucleolar stresses that perturb ribosome biogenesis (2). Indeed, RPL11 contributes to enhanced p53 stability and transcriptional activity in response to nucleolar stress and impaired ribosome biogenesis by binding and inhibiting the ubiquitin ligase activity of MDM2 (2-9). In addition to regulating p53 activity, research studies have also shown that RPL11 can inhibit cell cycle progression and ribosome biogenesis in response to nucleolar stress through repression of c-Myc transcriptional activity as well as its mRNA translation (10-12). Mutations in the gene have been found in patients with Diamond-Blackfan anemia (13). Alternate Names 60S ribosomal protein L11; cell growth-inhibiting protein 34; CLL-associated antigen KW-12; DBA7; GIG34; L11; Large ribosomal subunit protein uL5; ribosomal protein L11; RL11; RPL11; uL5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG