CST, 18596T, SIX2 (E7C3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SIX2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SIX2 (E7C3J) Rabbit Monoclonal Antibody (CST #18596) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SIX2 (E7C3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total SIX2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SIX2 protein. Background Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in mutant screens for embryonic eye development genes (1-3). The prototypical family member ( , ) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans ( ) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7). SIX2 is functionally important for the development of multiple tissues, notably the kidney, where it has been shown that SIX2 functions downstream of HOXA2 to maintain metanephric mesenchymal progenitors in an undifferentiated state, allowing for self-renewal of renal progenitor cells (8-10). Aberrant expression of SIX2 has likewise been identified as a potential contributing factor in the development of renal malignancies, including Wilms' Tumor (11-13). More recently, SIX2 has been shown to promote the maturation and maintenance of pancreatic beta cells (14,15). Alternate Names Homeobox protein SIX2; sine oculis homeobox homolog 2; SIX homeobox 2; SIX2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG