CST, 18657W2, Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying Tyrosine Hydroxylase. Highly specific and rigorously validated in-house, Tyrosine Hydroxylase (E2L6M) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 555 Conjugate) (CST #18657) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Tyrosine Hydroxylase (E2L6M) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human tyrosine hydroxylase protein. Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). Alternate Names dystonia 14; DYT14; DYT5b; HTH-2; TH; TH2; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG