CST, 18665S, ATF-3 (E9J4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATF-3. Validated for WB,IP,IF,F,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ATF-3 (E9J4N) Rabbit Monoclonal Antibody (CST #18665) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 46853 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP Specificity / Sensitivity ATF-3 (E9J4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human ATF-3 protein. Background Activating transcription factor 3 (ATF-3) is a basic leucine zipper-type transcription factor belonging to the ATF/cAMP responsive element binding protein family. ATF-3 can form homodimers or heterodimers with other family members and depending on the cell types and promoter context, it can suppress or activate the transcription of its target genes (1,2). Expression of ATF-3 is induced by a variety of factors including cytokines, genotoxic agents, and physiological stress. For example, both metformin and high-density lipoprotein (HDL) can induce ATF-3 expression in macrophages (1,3-5). Research studies also indicate that ATF-3 can function as an oncogene or a tumor suppressor depending on the context (6,7). Alternate Names activating transcription factor 3; ATF-3; ATF3; cAMP-dependent transcription factor ATF-3; Cyclic AMP-dependent transcription factor ATF-3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 23, 24 Source/Isotype: Rabbit IgG