CST, 18759S, Rab29 (E4P6M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAB7L1/RAB29. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Rab29 (E4P6M) Rabbit Monoclonal Antibody (CST #18759) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Rab29 (E4P6M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab29 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Rab29 protein. Background Rab29 (Rab7L1) is a member of the Ras GTPase superfamily of small monomeric G proteins responsible for regulation of intracellular membrane trafficking (1,2). Rab29 is involved in maintaining the integrity of the trans-Golgi network (TGN) and has been shown to play a role in the retrograde trafficking of mannose-6-phosphate receptors (M6PR) to the TGN (3). Rab29 is a substrate of LRRK2, a kinase associated with Parkinson's disease (PD), and is one of five genes encoded within the PD-linked PARK16 locus (4,5). It has been shown that Rab29 recruits LRRK2 to the TGN and stimulates its kinase activity. Mimicking LRRK2 phosphorylation of Rab29 at Thr71 and Ser72 appears to suppress activation of LRRK2. Thus, Rab29 acts as a regulator of LRRK2, controlling its activation and localization (6). Alternate Names DKFZp686P1051; Rab-7-like protein 1; RAB29; RAB29, member RAS oncogene family; RAB7, member RAS oncogene family-like 1; RAB7L; RAB7L1; Ras-related protein Rab-29; Ras-related protein Rab-7L1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG