CST, 18772S, Cathepsin D (E7Z4L) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying Cathepsin D mouse. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cathepsin D (E7Z4L) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #18772) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cathepsin D (E7Z4L) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse cathepsin D protein. Background Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9). Alternate Names Cat; CatD; Cathepsin D; CD; Ctsd; OTTMUSP00000020357 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG