CST, 18799S, Vinculin (E1E9V) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying vinculin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Vinculin (E1E9V) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #18799) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Vinculin (E1E9V) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total vinculin protein. This antibody conjugate also reacts with metavinculin, a 145 kDa splice variant of vinculin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human vinculin protein. Background Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region, and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed conformation. The open and active form of vinculin translocates to focal adhesions, where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6). This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin, and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8). This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9). Alternate Names CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; meta-vinculin; Metavinculin; MV; MVCL; VCL; VINC; Vinculin; Vinculin iso2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 124 Source/Isotype: Rabbit IgG